Realtime PCR법에 의한 청소년의 치아우식 유발세균 감염률 조사
Determination of the infection rate of dental caries-causing bacteria in adolescents by a real-time PCR test method
문예림, 박지현, 조자원, 김우람,
소속 상세정보
문예림 ( Mun Ye-Rim ) - Dankook University Graduate School of Public Health & Social Welfare Department of Oral Health
박지현 ( Park Ji-Hyeon ) - Gangneng Yeongdong University Department of Dental Hygiene
조자원 ( Cho Ja-Won ) - Dankook University College of Dentistry Department of Preventive Dentistry
김우람 ( Kim Woo-Ram ) - Dankook University College of Dentistry Department of Preventive Dentistry
Abstract
Objectives: The aim of this study was to investigate the infection rates of bacteria associated with dental caries among children and adolescents in Korea.
Methods: Oral examinations were conducted in 146 students. The numbers of bacteria associated with dental caries, such as Streptococcus mitis, Streptococcus mutans, Streptococcus sobrinus, and Lactobacillus casei, in the subjects’ oral cavity were counted by real-time PCR, and infection rates for those bacteria were additionally investigated.
Results: The infection rate of Streptococcus mitis, Streptococcus mutans, Streptococcus sobrinus, and Lactobacillus casei was 99.3% (145/146), 63.0% (92/146), 1.4% (2/146), and 25.3% (37/146), respectively. The amounts of Streptococcus sobrinus and Lactobacillus casei did not differ with the participants’ age. However, the number of Streptococcus mutans was 537.6 for primary school students, 5698.2 for middle school students, and 19037.5 for high school students. The mean number of oral bacteria increased with age (P<0.05). The mean bacterial numbers of the infected subjects indicated significant differences in the numbers of Streptococcus mitis and Streptococcus mutans (P<0.05).
Conclusions: The infection rates of Streptococcus mitis and Streptococcus mutans were distinct in children and adolescents. Efforts to control the bacteria associated with dental caries are needed to prevent dental caries.
키워드
Infection rate; Oral microorganism; Streptococcus mutans
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